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Welcome to the website of Prof Ute Roessner’s lab group at the School of BioSciences, University of Melbourne.

Our main research interest is the development and application of metabolomics technologies to study metabolism and physiology of biological systems. Metabolomics, defined as the comprehensive and quantitative analysis of all metabolites in a biological system, is a multidisciplinary approach combining analytical biochemistry, biology and bioinformatics. In particular, we are interested in understanding how cereal crops adapt and tolerate harsh environmental situations such as water deficiency or salinity. For instance, we compare the metabolic responses of wheat or barley cultivars differing in their tolerance levels to drought or salt stress aiming to identify the tolerance mechanisms.

As part of Ute Roessner’s ARC Future Fellowship, we are studying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley (Hordeum vulgare).

At Metabolomics Australia, we use state-of-the-art mass spectrometry technologies aiming to offer metabolomics services to Australian researchers and industry partners which includes method development for the analysis of metabolites of interest, experimental design, application of the appropriate sample preparation right through to data analysis, data mining and statistical analysis. We have provided metabolomics services to hundreds of collaborators and clients from a diverse range of research areas. Projects run in this group are addressing questions in plant, microbial, mammalian research as well for biomedical and agricultural applications.

In all groups we welcome Hons/Msc and PhD students willing to be part of the developments and applications of the fairly young scientific area of metabolomics.

Examples of projects:

Identifying novel salinity tolerance mechanisms by spatial and temporal analysis of lipids in barley (ARC Future Fellowship, PhD projects of Dingyi Yu and Daniel Sarabia)
Control points in nitrogen uptake: enhancing the response of maize to nitrogen supply and demand (ARC Linkage, Investigators Garnett T, S Heuer, M Small, U Roessner, A Rafalski, K Dhugga, H Kuchel)
Small molecules with large effect: Metabolites for stress tolerance and nutrient recycling (ARC Linkage, Investigators Heuer S, Roessner U, Gupta R, Okamoto M, Hoefgen R)
Coral Health: Bleaching and Disease (Great Barrier Reef Foundation. Investigators Leggat B, Ainsworth T, Rosenthal N, Gordon B, Roessner U)
Identification of metabolite markers in frost tolerance in wheat (Investigators Dolferus R, Roessner U, PhD project of Bo Eng Cheong)
Determine the biochemical basis of root-microbe interactions in salt stressed barley (PhD project Sneha Gupta)
Determine the effect of temperature stress on root-microbe interactions in Brachypodium (Investigators Watt M, Arsova B, Roessner U, PhD project Martino Schillaci)
The impact of nutrient imbalances on cell wall biosynthesis (Investigators Persson S, Hoefgen R, Roessner U, PhD project Mike Ogden)
The Australian Plant Lipidomics Centre (Investigators Roessner U, Taylor N, Rupasinghe T, Bradley D, Hennessy T)

We are using mass spectrometry coupled to chromatographic separation for metabolomics.

The following instruments are in our facility:

GC-Q-MS (Agilent 7890 GC coupled 5975 single quadrupole MS)
GC-QQQ-MS (Agilent 7890 GC coupled to 7000 triple quadrupole MS)
GC-QQQ-MS (Agilent 7890 GC coupled to 7010A triple quadrupole MS)
LC-QTOF-MS (Agilent 1200 series LC coupled to 6520 QTOF MS)
LC-QTOF-MS (Agilent 1290 series LC coupled to a 6545 QTOF MS)
LC-QQQ-MS (Agilent 1200 series LC coupled to 6420 triple quadrupole MS)
LC-QQQ-MS (Agilent 1290 LC coupled to 6490 triple quadrupole MS)
LC-QQQ-MS (Agilent 1290 LC coupled to 6470 triple quadrupole MS)
LC-Triple TOF-MS (Agilent 1290 LC coupled to a Sciex 6600 Triple TOF MS)
MALDI-FT-ICR-MS (Bruker SolariX 7T FT-ICR-MS with a MALDI and ESI source)

The following methods are used for our research:

GC-MS based metabolite profiling using GC-Q-MS
GC-MS based fatty acid profiling using GC-Q-MS
GC-MS based metabolite quantification using GC-QQQ-MS
LC-MS based metabolite profiling using LC-QTOF-MS
LC-MS based metabolite quantification using LC-QQQ-MS
LC-MS based lipid profiling using LC-QTOF and LC-Triple TOF MS
LC-MS based lipid quantification using LC-QQQ-MS
MALDI based metabolite and lipid imaging using FT-ICR-MS
MALDI based lipid analysis using TLC-MALDI-FT-ICR-MS
Metabolite identification using MS, MS/MS and MSn

Principal Investigator

Ute Roessner

Postdoctoral Fellows

Siria Natera
Berin Boughton
Adrian Lutz
Thusita Rupasinghe

Research Assistant

Himasha Mendis
Roshan Cheetamun
Veronica Lui
Kim Kwan

Students

PhD

Dingyi Yu
Daniel Sarabia
Sneha Gupta
Bo Eng Cheong
Martino Schillaci
Marianne Weisser
Tannaz Zare
Cheka Kehelpannala
Allene Macabuhray
Mike Ogden

Masters

Brayden Fraser

Honours

Holly Khoury

Former lab members

Jairus Bowne
Damien Callahan
Daniel Dias
Camilla Hill
Alice Ng
Terra Stark
Priyanka Reddy
Niru Jayasinghe
Natalie Pereira
William Ho

Books

Roessner U, Dias D (Eds) (2013) Metabolomics Tools for Natural Product Discoveries . In Methods in Molecular Biology Series. Humana Press USA
Roessner U (Ed) (2012) InTech Open Access Publisher
Villas-Boas SG, Roessner U, Hansen M, Smedsgaard J, Nielsen J (Eds) (2007) Metabolome Analysis. Wiley & Sons, New Jersey, NJ, USA

Book chapters

Dayalan S, Xia J, Soicer RA, Salek R, Roessner U (2018) Metabolome analysis. In: Encyclopedia of Bioinformatics and Computational Biology. Elsevier
Jayasinghe NS, Mendis H, Roessner U, Dias U (2018) Quantification of sugars and organic acids in biological matrices using gas chromatography-triple quadrupole-mass spectrometry (GC-QqQ-MS). In: Plant Metabolomics. Antonio C (ed). In Methods in Molecular Biology Series. Humana Press USA
Rupasinghe, TWT, Roessner U (2018) Untargeted plant lipidomics. In: Plant Metabolomics. Antonio C (ed). In Methods in Molecular Biology Series. Humana Press USA
Rupasinghe TWT, Roessner U (2016) Lipid extraction. In Encyclopaedia of Lipidomics. Wenk M (Ed). Springer
Hill, B., Roessner, U (2015) Advances in high-throughput LC-MS analysis for plant metabolomics. In Advanced LC-MS applications for metabolomics, De Vooght-Johnson, R (ed.), Future Science Ltd.
Hill CB, Dias DA, Roessner U (2015) Current and emerging applications in the field of agricultural biotechnology. In Advances in Food Biotechnology, Ravishankar, R (ed.), John Wiley & Sons Ltd.
Hill CB, Bacic A, Roessner U (2014) LC-MS profiling to link metabolic and phenotypic diversity in plant mapping populations.In Mass Spectrometry Methods in Metabolomics, Humana Press, NY, USA, Vol. 1198, pp. 29-41
Roessner U, Dias D (2013) Plant tissue extraction for metabolomics. In: Metabolomics Tools for Natural Product Discoveries. Roessner U and Dias D (eds). In Methods in Molecular Biology Series. Humana Press USA
Hill CB, Roessner U (2013) Metabolic profiling of plant by GC-MS. In: GC-MS Metabolomics Handbook. Weckwerth W (ed). Springer, Heidelberg, Germany
Roessner U, Beckles DM (2012) Metabolomics for salinity research. In: Plant Salinity Tolerance. Shabala S (ed). In: Methods in Molecular Biology Series 913:203-215. Humana Press USA
Beckles D, Roessner U (2011) Plant Metabolomics – Applications and opportunities for agricultural biotechnology. In Plant biotechnology and agriculture: Prospects for the 21^st century, Altmann A, Hasegawa PM (eds), Elsevier
Roessner U, Nahid A, Hunter A, Bellgard M (2011) Metabolomics – the combination of analytical chemistry,biology and informatics. In: Moo-Young M, (ed.) Comprehensive Biotechnology, Second Edition, vol. 1, pp. 447-459. Elsevier
Bowne J, Bacic A, Tester M, Roessner U (2011) Abiotic stress and Metabolomics. In: Annual Plant Reviews Volume 43, Biology of Plant Metabolomics, First Edition. Hall R (ed), pp 61–85, Blackwell Publishing
Gresshoff PM, Indrasumunar A, Miyahara A, Nontachaiyapoom S, Biswas B, Lin Y-H, Lin M-H, Reid D, Callahan D, Capon R, Zhang H, Jiang Q, Hirani T, Kobe B, Men A, Hayashi S, Scott PT, Kereszt A, Miyagi M, Li DX, Chan PK, Roessner U, Djordjevic MA, Kinkema M, Ferguson B (2009) Functional genomic analysis of systemic cell division regulation in legumes. In: Shu QY (ed.) Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations. Rome , 2009, 399-402
Roessner U, Beckles DM (2009) Metabolite measurements. In Plant Metabolic Networks, Junker B and Schwender J, eds., Springer, Heidelberg, Germany
Roessner-Tunali U (2007) Uncovering the plant metabolome: current and future challenges. In „Concepts in Plant Metabolomics.“ Nikolau BJ and Wurtele ES, eds., Springer, Dortrecht, The Netherlands
Tarpley L, Roessner U (2007) Metabolomics: Enablingsystems-level phenotyping in rice functional genomics. In Rice Functional Genomics- Challenges, Progress and Prospects, Upadhyaya NM, ed., Springer, Heidelberg, Germany, pp 91-108
Roessner U, Pettolino F (2007) The importance of anatomy and physiology in plant metabolomics. In “Metabolomics” of the book series Trends in Current Genetics, Jewett MC and Nielson J, eds., Springer, Heidelberg, Germany
Fernie AR, Roessner U, Willmitzer L (2002) Metabolic profiling of plant cells. Web essay 11.2 in Taiz L and Zeiger E „Plant Physiology“, 3. Edition, Sinauer Associates, Inc., Publisher

Reviews

Boughton BA, Roessner U (2016) Imaging mass spectrometry for spatial metabolomics. Austral Biochem 43, 9-12
Boughton BA, Thinagaran D, Sarabia D, Bacic A, Roessner U (2016) Mass spectrometry imaging for plant biology: A review. Phytochem 15, 455-488
Hill CB, Czauderna T, Klapperstück M, Roessner U, Schreiber F (2015) Metabolomics, standards and metabolic modeling for synthetic biology in plants. Frontiers Bioeng Biotechnol 3, 16, doi: 10.3389/fbioe.2015.00167
Jones OAH, Dias DA, Callahan DL, Kouremenos KA, Beale DJ, Roessner U (2015) The use of metabolomics in the study of metals in biological systems. Metallomics 7, 29-38
Cogger VC, Roessner U, Warren A, Fraser R, Le Couteur DG (2013) A sieve-raft hypothesis for the regulation of endothelial fenestrations. Computational and Structural Biotechnology Journal 8, e201309003
Shelden MC, Roessner U (2013) Advances in functional genomics for investigating salinity stress tolerance mechanisms in cereals. Frontiers in Plant Science 4, 123, doi: 10.3389/fpls.2013.00123
Dias D, Urban S, Roessner U (2012) A historical overview of natural products in drug discovery. Metabolites 2, 303-336
Roessner U, Bacic A (2009) Metabolomics in plant research. Australian Biochemist, Vol 40 No 3, 9-13
Roessner U, Bowne J (2009) What is metabolomics all about? Biotechniques 46:363-365
Sanchez DH, Siahpoosh MR, Roessner U, Udvardi U, Kopka J (2008) Plant metabolomics reveals conserved and divergent metabolic responses to salinity. Physiol Plantarium 132:209-219
Fiehn O, Sumner L, Ward J, Rhee SY, Dickerson J, Lange M, Lane G, Roessner U, Last R, Nikolau B (2007) Minimum reporting standards for plan biology context in metabolomic studies. Metabolomics 3, 195-201
Jenkins H, Hardy N, Beckmann M, Draper J, Smith AR, Taylor J, Fiehn O, Goodacre R, Bino RJ, Hall R, Kopka J, Lane GA, Lange BM, Liu JR, Mendes P, Nikolau BJ, Oliver SG, Paton NW, Rhee S, Roessner-Tunali U, Saito K, Smedsgaard J, Sumner LW, Wang T, Walsh S, Syrkin Wurtele E, Kell DB (2004) A proposed framework for the description of plant metabolomics experiments and their results. Nature Biotech. 22, 1601-1606
Bino RJ, Hall RH, Fiehn O, Kopka J, Saito K, Draper J, Nikolau B, Mendes P, Roessner-Tunali U, Beale M, Trethewey RN, Lange BM, Syrkin Wurtele E, Sumner L (2004) Opinion: Potential of metabolomics as a functional genomics tool. Trends Plant Sci., 9, 418-425
Roessner U, Willmitzer L, Fernie AR (2002) Metabolic profiling and biochemical phenotyping of plant systems. Plant Cell Rep. 21, 189-196

In press and published

Hillyer K, Dias DA, Lutz A, Roessner U, Davy S (2018) ¹³C metabolomics reveals widespread change in carbon fate during coral bleaching. Metabolomics (in press)
Luengwilai K, Beckles D, Roessner U, Dias DA, Lui V, Siriphanich J (2018) Identification of physiological changes and key metabolites coincident with postharvest internal browning of pineapple (Ananas comosus) fruit. Postharvest Biology and Technology (accepted Oct 2017)
Summers M, Rupasinghe T, Vasiljevski ER, Evesson FJ, Mikulec K, Peacock L, Quinlan KG, Cooper S, Roessner U, Stevenson DA, Little D, Schindler A (2017) Dietary intervention rescues myopathy associated with neurofibromatosis type 1. Human Molecular Genetics (in press)
Soll C, Silva T, Grossi-de-Sa MF, Bacic A, Boughton BA, Oldach K, Callahan DL, Roessner U, Carneiro R, Pelegrini P, Polez V, Firmino A, Sheedy J, Rocha E, Silva L (2017) Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita. Biotechnology Research and Innovation (in press)
Matthews JL, Crowder CM, Oakley CA, Lutz A, Roessner U, Meyer E, Grossman AR, Weis VM2, Davy SK (2017) Optimal nutrient exchange and immune responses operate in partner specificity in the cnidarian-dinoflagellate symbiosis. PNAS (in press, doi/10.1073/pnas.1710733114)
Hillyer KE, Dias DA, Lutz A, Wilkinson SP, Roessner U, Davy SK (2017) Mapping carbon fate during bleaching in a model cnidarian symbiosis: the application of ¹³C metabolomics. New Phytologist 214, 1551-1562
Selby-Pham JM, Lutz A, Moreno-Moyano LT, Boughton BA, Roessner U, Johnson AAT (2017) Diurnal changes in transcript and metabolite levels during the iron deficiency response of rice. Rice 10:14. doi: 10.1186/s12284-017-0152-7
Barbosa GB, Jayasinghe NS, Natera SH, Inutan ED, Peteros NP, Roessner U (2017) From common to rare Zingiberaceae plants – A metabolomics study using GC-MS. Phytochemistry 140, 141-150
Kiani-Pouya A, Roessner U, Jayasinghe NS, Lutz A, Rupasinghe T, Bohm J, Hedrich R, Shabala S (2017) Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species. Plant Cell Environment 40, 1900-1915
Hunter A, Dayalan S, De Souza D, Power B, Lorrimar R, Szabo T, Nguyen T, O’Callaghan S, Hack J, Pyke J, Nahid A, Barrero R, Roessner U, Likic V, Tull D, Bacic A, McConville M, Bellgard M (2017) MASTR-MS: a web-based collaborative laboratory information management system (LIMS) for metabolomics. Metabolomics 13:14 DOI 10.1007/s11306-016-1142-2
Sabermanesh K, Holtham LR, George J, Roessner U, Boughton BA, Heuer S, Tester M, Plett DC, Garnett TP (2017) The transition from a maternal to external nitrogen source in maize seedlings. J Integrative Plant Biol 59, 261-274
Jarvis D, Ho Y, Lightfoot D, Schmöckel S, Li B, Michell C, Kharbatia G, Saber N, Woo Y, Gao G, Sharp A, Borm T, Schijlen E, Mineta K, Ohyanagi H, Dally N, Jung C, Boughton B, Roessner U, Gehring C, Murphy K, Gojobori T, van der Linden G, van Loo R, Jellen E, Maughan P, Negrão S, Tester M (2017) The genome of Chenopodium quinoa. Nature 542, 307-312
Cao D, Lutz A, Hill CB, Callahan DL, Roessner U (2017) A quantitative profiling method of phytohormones and other metabolites applied to barley roots subjected to salinity stress. Frontiers in Plant Science (doi: 10.3389/fpls.2016.02070)
Hillyer KE, Dias DA, Lutz A, Wilkinson SP, Roessner U, Davy SK (2016) Metabolite profiling of symbiont and host during thermal stress and bleaching in the coral Acropora aspera. Coral Reef (DOI 10.1007/s00338-016-1508-y)
Shabala L, Zhang J, Pottosin I, Bose J, Zhu M, Fuglsang AT, Velarde-Buendia A, Massart A, Hill CB, Roessner U, Bacic A, Wu H, Azzarello E, Pandolfi C, Zhou M, Poschenrieder C, Mancuso S, Shabala S (2016) Cell-type specific K⁺ retention and H⁺-ATPase activity protect barley (Hordeum vulgare) roots from salinity stress. Plant Physiol 172, 2445-2458
Sroka WD, Boughton BA, Reddy P, Roessner U, Słupski P, Jarzemski P, Markuszewski MJ, Marszałł MP (2016) Determination of amino acids in urine of patients with prostate cancer and benign prostate growth. European J Cancer Prevention DOI: 10.1097/CEJ.0000000000000248)
Hill CB, Cassin A, Keeble-Gagnère, Doblin MS, Bacic A, Roessner U (2016) De novo transcriptome assembly and analysis of differentially expressed genes of two barley genotypes reveal root-zone-specific responses to salt exposure. Scientific Rep 6:31558 (http://dx.doi.org/10.1038/srep31558)
Plett D, Holtham L, Baumann U, Kalashyan E, Francis K, Enju A, Toubia J, Roessner U, Bacic A, Rafalski A, Dhugga K, Tester M, Garnett T, Kaiser B (2016) Nitrogen assimilation system in maize is regulated by developmental and tissue-specific mechanisms. Plant Mol Biol 92, 293-312 (DOI 10.1007/s11103-016-0512-5)
Shelden MC, Dias DA, Jayasinghe NS, Bacic A, Roessner U (2016) Root spatial metabolite profiling of two contrasting barley genotypes (Hordeum vulgare) in response to short-term salt stress. J Exp Bot 67, 3731-3745
Wang Y, Lyu W, Berkowitz O, RadomiljaC JD, Law SR, Murcha MW, Carrie C, Teixeira PF, Kmiec B, Duncan O, Van Aken O, Narsai R, Glaser E, Huang S, Roessner U, Millar AH, Whelan J (2016) Inactivation of mitochondrial complex I induces the expression of a twin-cysteine protein that targets and affects cytosolic, chloroplastidic and mitochondrial function. Mol Plant 9, 696-710
Natera SHA, Hill CB, Rupasinghe TWT, Roessner U (2016) Salt-stress induced alterations in the root lipidome of two barley genotypes with contrasting responses to salinity. Funct Plant Biol 43, 207-219
Williams R, Dias DA, Jayasinghe N, Roessner U, Bennett L (2016) Beta-glucan-depleted, glycopeptide-rich extracts from Brewer’s and Baker’s yeast (Saccharomyces cerevisiae) lower interferon-gamma production by stimulated human blood cells in vitro. Food Chem 197 (Part A), 761-768
Callahan DC, Hare DJ, Bishop DP, Doble PA, Roessner, U (2016) Elemental imaging of leaves from the metal hyperaccumulating plant Noccaea caerulescens shows different spatial distribution of Ni, Zn and Cd. RSC Advances 6, 2337 – 2345
Pyke JS, Callahan DL, Bacic A, McConville MJ, Roessner U (2015) A tandem liquid chromatography–mass spectrometry (LC–MS) method for profiling small molecules in complex samples. Metabolomics 11, 1552-1562
Wijetunge CD, Saeed I, Boughton BA, Roessner U, Halgamuge SK (2015) A new peak detection algorithm for MALDI mass spectrometry data base on Asymmetric Pseudo-Voigt model. BMC Genomics 6 (Suppl X), S12
Dias DA, Hill CB, Jayasinghe NS, Atieno J, Sutton T, Roessner U (2015) Quantitative profiling of polar primary metabolites of two chickpea cultivars with contrasting responses to salinity. J Chromat B 1000, 1-13
Wijetunge CD, Saeed I, Boughton BA, Spraggins JM, Caprioli RM, Bacic A, Roessner U, Halgamuge SK (2015) EXIMS: an improved data analysis pipeline based on a new peak picking method for EXploring Imaging Mass Spectrometry data. Bioinformatics (doi: 1093/bioinformatics/btv356)
Boughton BA, Reddy P, Boland MP, Roessner U , Yates P (2015) Non-protein amino acids in Australian acacia seed: Implications for food security and recommended processing methods to reduce djenkolic acid. Food Chemistry 179, 109-115
Roessner U, Bearden DW , Ebbels T (2015) The international Metabolomics Society in 2015: the path forward to success. Metabolomics 11 (1), 1-2
Hill CB, Taylor JD, Edwards J, Mather D, Langridge P, Bacic A, Roessner U (2015) Detection of QTL for metabolic and agronomic traits in wheat with adjustments for variation at genetic loci that affect plant phenology.Plant Science 233, 143–154
Gholami M, Boughton BA, Fakhari AR, Ghanati F, Mirzaei HH, Borojeni LY, Zhang Y, Breitbach ZS, Armstrong DW, Roessner U (2013) Metabolomic study reveals a selective accumulation of L-arginine in the D-ornithine treated tobacco cell suspension culture. Process Biochemistry49, 140-147
Aizat WM, Dias DA, Stangoulis JCR, Able JA, Roessner U, Able AJ (2013) Metabolomics of capsicum ripening reveals modification of the ethylene pathway and carbon metabolism. Postharvest Biology and Technology 89, 19-31
Pavagadhi S, Natera S, Roessner U, Balasubramanian R (2013) Insights into lipidomic perturbations in zebrafish tissues upon exposure to microcystin-LR and microcystin-RR. Environmental Science Technology DOI: 10.1021/es4004125
Wijetunge CD, Li Z, Saeed I, Bowne J, Hsu AL, Roessner U, Bacic A, Halgamuge SK (2013) Exploratory analysis of high-throughput metabolomic data . Metabolomics 9,1311-1320 (doi: 10.1007/s11306-013-0545-6)
Liu D, Ford KL, Roessner U, Natera S, Cassin AM, Patterson JH, Bacic A (2013) Rice suspension cultured cells are evaluated as a model system to study salt responsive networks in plants using a combined proteomic and metabolomic profiling approach. Proteomics 12-13, 2046-2062
Hill CB, Taylor JD, Edwards J, Mather D, Bacic A, Langridge P, Roessner U (2013) Whole genome mapping of agronomic and metabolic traits to identify novel quantitative trait loci in bread wheat (Triticum aestivum) grown in a water-limited environment. Plant Physiology 162, 1266-1281
Shelden MC, Roessner U, Tester M, Bacic T (2013) Genetic variation in the root growth response of barley cultivars to salinity stress. Functional Plant Biology 40, 516-530
Hill CB, Jha D, Bacic A, Tester M, Roessner U (2013) Characterization of ion contents and metabolic responses to salt stress of different AtHKT1;1 genotypes and their parental strains in Arabidopsis thaliana. Molecular Plant 6, 350-368
Garnett T, Conn V, Plett D, Conn S, Zanghellini J, Mackenzie N, Enju A, Francis K, Holtham L, Roessner U, Bacic A, Shirley N, Rafalski A, Dhugga K, Tester M , Kaiser BN (2013) The response of the maize nitrate transport system to nitrogen demand and supply across the lifecycle. New Phytologist 198, 82-94
Kabir AH, Paltridge NG, Roessner U, Stangoulis JCR (2013) Mechanisms associated with Fe-deficiency tolerance and signalling in shoots of Pisum sativum Physiologia Plantarum (doi: 10.1111/j.1399-3054.2012.01682.x)
De Livera AM, Dias DA, De Souza DP, Rupasinghe T, Pyke J, Tull D, Roessner U, McConville M, Speed TP (2012) Normalising and integrating metabolomics data. Analytical Chemistry 84, 10768-10776
O’Callaghan S, DeSouza DP, Isaac A, Wang Q, Hodkinson L, Olshansky M, Erwin T, Appelbe B, Tull DL, Roessner U, Bacic A, McConville MJ, Likic VA (2012) PyMS: a Python toolkit for processing of gas chromatography-mass spectrometry (GC-MS) data. Application and comparative study of selected tools. BMC Bioinformatics 13, 115
Temmerman L, De Livera AM, Bowne JB, Sheedy JR, Callahan DL, Nahid A, De Souza DP, Schoofs L, Tull DL, McConville MJ, Roessner U, Wentworth JM (2012) Cross-platform urine metabolomics of experimental hyperglycemia in type 2 diabetes. Journal of Diabetes and Metabolism. S:6
Callahan DL, Roessner U, Dumontet V, Livera AMD, Doronilla A, Baker AJM, Kolev SD. (2012) Elemental and metabolite profiling of nickel hyperaccumulators from New Caledonia. Phytochem 81, 80-89
Bowne JB, Erwin TA, Juttner J, Schnurbusch T, Langridge P, Bacic A, Roessner U (2012) Drought responses of wheat leaf tissues at the metabolite level. Molecular Plant 5, 418-429
Boughton BA, Callahan DL, Silva C, Bowne J, Nahid A, Rupasinghe T, Tull DL, McConville MJ, Bacic A, Roessner U (2011) Comprehensive profiling and quantitation of amine group containing metabolites. Anal Chem. 83, 7523-7530
Gruber BD, Delhaize E, Richardson AE, Roessner U, James RA, Howitt SM, Ryan PR (2011) Characterisation of HvALMT1 function in transgenic barley plants. Functional Plant Biology 38, 163-175
Lukman AI, Gong B, Marjo CE, Roessner U, Harris AT (2011) Facile synthesis,stabilization, and anti-bacterial performance of discrete Ag nanoparticles using Medicago sativa seed exudates. J Colloid Interface Sci 353, 433–444
Rao SR, Ford KL, Cassin AM, Roessner U, Patterson JH, Bacic A. (2010) Proteomic and metabolic profiling of rice suspension culture cells as a model to study abscisic acid signaling response pathways in plants. J Proteome Res. 9, 6623-6634
Villas-Bôas SG, Roessner U (2009) 1st Australasian Symposium on Metabolomics. Bioanalysis, 1521-1522
Kibble NAJ, Mehdi Sohani M, Shirley N, Byrt C, Roessner U, Bacic A, Schmidt O, Schultz CJ (2009) Phylogenetic analysis and functional characterisation of strictosidinesynthase-like genes in Arabidopsis thaliana. Functional Plant Biology 36, 1098-1109
Widodo, Patterson JH, Newbigin E, Tester M, Bacic A, Roessner U (2009) Metabolic responses to salt stress of barley (Hordeum vulgare) cultivars, Sahara and Clipper, which differ in salinity tolerance. J Exp Bot 60, 4089-4103
Callahan DL, DeSouza D, Bacic A, Roessner U (2009) Profiling of polar metabolites in biological extracts using diamondhydride-based aqueous normal phase chromatography. J Sep Sci 32, 2273-2280
Callahan DL, Roessner U, Dumontet V, Perrier N, Wedd AG, O’Hair RAJ, Baker AJM, Kolev SD (2008) LC-MS and GC-MS metabolite profiling of nickel(II) complexes in the latex ofnickel- hyperaccumulating tree Sebertia acuminata and identification of methylated aldaric acid as a new nickel(II) ligand. Phytochem 69, 240-251
Huang CY, Roessner U, Eickmeier I, Genc Y, Callahan DL, Shirley N, Langridge P, Bacic A (2008) Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare ). Plant Cell Physiol 49, 691-703
Pereira-Netto AB, Roessner U, Fujioka S, Bacic A, Asami T, Yoshida S, Clouse SD. (2008) Shooting control by brassinosteroids: metabolomic analysis and effect of brassinazole on Malus prunifolia, the Marubakaido apple rootstock. Tree Physiol. 29, 607-620
Stamova BS, Roessner U, Suren S, Laudencia-Chingcuanco D, Bacic A, Beckles DM (2008) Metabolic profiling of transgenic wheat over-expressing the high- molecular-weight Dx5 glutenin subunit. Metabolomics 5, 239-350
Glassop D, Roessner U, Bacic A, Bonnett G (2007) Changes in the sugarcane metabolome with stem development. Are they related to sucrose accumulation? Plant Cell Physiol. 48, 573-584
Semel Y, Schauer N, Roessner U, Zamir D, Fernie AR (2007) Metabolite analysis for the comparison of irrigated and non-irrigated field grown tomato of varying genotype. Metabolomics 3, 289-295
Jacobs A, Lunde C, Bacic A, Tester M, Roessner U (2007) The impact of constitutive expression of a moss Na+transporter on the metabolomes of rice and barley. Metabolomics 3, 307-317
Schauer N, Semel Y, Roessner U, Gur A, Balbo I, Carrari F, Pleban T , Perez-Melis A, Bruedigam C, Kopka J, Willmitzer L, Zamir D, Fernie AR (2006) Comprehensive metabolic profiling and phenotyping of interspecific introgression lines for tomato improvement. Nature Biotech. 24, 447-454
Roessner U, Patterson JH, Forbes MG, Fincher GB, Langridge P, Bacic A (2006) An investigation of boron toxicity in barley using metabolomics. Plant Physiol. 142, 1087-1101
Hosp J, Tashpulatov A, Roessner U, Barsova E, Katholnigg H, Steinborn R, Melikant B, Lukyanov S, Heberle-Bors E, Touraev A (2006) Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores. Plant Mol Biol 63, 137-149
Schauer N, Steinhauser D, Strelkov S, Schomburg D, Allison G, Moritz T, Lundgren K, Roessner-Tunali U, Forbes MG, Willmitzer L, Fernie AR, Kopka J (2005) GC-MS libraries for the rapid identification of metabolites in complex biological samples. FEBS Lett. 579, 1332-1337
Gass N, Glagotskaia T, Mellema S, Barone M, Mandel T, Roessner-Tunali U, Kuhlemeier C (2005) Pyruvate decarboxylase provides growing pollen tubes with a competitive advantage in petunia. Plant Cell 17, 2355-2368
Roessner-Tunali U, Lui J, Leisse A, Balbo I, Perez-Melis A, Willmitzer L, Fernie AR (2004) Flux analysisof organic and amino acid metabolism in potato tubers by gas chromatography-mass spectrometry following incubation in ¹³C labelled isotopes. Plant J., 39, 668-679
Roessner-Tunali U, Hegemann B, Lytovchenko A, Carrari F, Bruedigam C, Granot D, Fernie AR (2003) Metabolic profiling of transgenic tomato plants overexpressing hexokinase reveals that the influence of hexose phosphorylation diminishes during fruit development. Plant Physiol., 133, 84-99
Roessner-Tunali U, Urbanczyk-Wochniak E, Czechowski T, Kolbe A, Willmitzer L, Fernie AR (2003) De novo amino acid biosynthesis in plant storage tissues is regulated by sucrose levels. Plant Physiol., 133, 683-692
Leggewie G, Kolbe A, Lemoine R, Roessner U, Lytovchenko A, Frommer WB, Riesmeier JW, Willmitzer L, Fernie AR (2003) Overexpression of the sucrose transporter SoSUT1 in potato results in alterations in leaf carbon partitioning and in sucrose export but has little impact on tuber metabolism. Planta, 217, 158-167
Giege P, Heazlewood JL, Roessner-Tunali U, Millar AH, Fernie AR, Leaver CJ, Sweetlove LJ (2003) Enzymes of glycolysis are functionally associated with the mitochondrion in Arabidopsis cells. Plant Cell, 15, 2140-2151
Urbanczyk-Wochniak E, Luedemann A, Kopka J, Selbig J, Roessner-Tunali U, Willmitzer L, Fernie AR (2003) Parallel analysis of transcript and metabolic profiles: a new approach in systems biology. EMBO Rep., 4, 989-992
Urbanczyk-Wochniak E, Leisse A, Roessner-Tunali U, Lytovchenko A, Riesmeier J, Willmitzer L, Fernie AR (2003) Expression of a bacterial glucose (xylose) isomerase in potato tubers results in an altered hexose composition and a consequent induction of metabolism. Plant Cell Physiol., 44, 1359-1367
Fernie AR, Tauberger E, Roessner U, Lytochenko A, Trethewey RN, Willmitzer L (2002) Antisense repression of cytosolic phosphoglucomutase in potato (Solanum tuberosum) results in a severe growth retardation, reduction in tuber number and altered carbon metabolism. Planta, 214, 510-520
Westram A, Lloyd JR, Roessner U, Riesmeier JW, Kossmann J (2002) Increases of 3-phosphoglyceric acid in potato plants through antisense reduction of cytoplasmic phosphoglycerate mutase impairs photosynthesis and growth, but does not increase starch contents. Plant Cell Environ., 25, 1133-1143
Kühn C, Hajirezaei M-R, Fernie AR, Roessner-Tunali U, Czechowski T, Hirner B, Frommer WB (2002) Tuber-specific antisense inhibition of the sucrose transporter StSUT1 in transgenic potato plants. Plant Physiol., 131, 102-113
Roessner U, Luedemann A, Brust D, Fiehn O, Linke T, Willmitzer L, Fernie AR (2001) Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell, 13, 11-29
Roessner U, Willmitzer L, Fernie AR (2001) High-resolution metabolic phenotyping of genetically and environmentally diverse plant systems – identification of phenocopies. Plant Physiol., 127, 749-764
Fernie AR, Roessner U, Geigenberger P (2001). The sucrose analog palatinose leads to astimulation of sucrose degradation and starch synthesis when supplied to discs of growing potato tubers (Solanum tuberosum). Plant Physiol., 125, 1967-1977
Farré EM, Tiessen A, Roessner U, Geigenberger P, Trethewey RN, Willmitzer L (2001) Analysis of the compartmentation of glycolytic intermediates, nucleotides,sugars, organic acids, amino acids and sugar alcohols in potato tubers using anon-aqueous fractionation method. Plant Physiol., 127, 685-700
Fernie AR, Roessner U, Leisse A, Lubeck J, Trethewey RN, Willmitzer L (2001) Simultaneous antagonistic modulation of enzyme activities in transgenic plants through the expression of a chimeric transcript. Plant Physiol. Biochem., 39, 825-830
Zeh M, Casazza AP, Kreft O, Roessner U, Bieberich K, Willmitzer L, Hoefgen R, Hesse H (2001) Antisense inhibition of threonine synthase leads to high methionine content in transgenic potato plants. Plant Physiol., 127, 792-802
Fernie AR, Roessner U, Trethewey RN, Willmitzer L (2001) The contribution of plastidial phosphoglucomutase to the control of starch synthesis within potato tubers. Planta, 213, 418-426
Roessner U, Wagner C, Kopka J, Trethewey RN, Willmitzer L (2000) Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry. Plant J., 23, 131-142
Edwards A, Fulton DC, Hylton CM, Jobling SA, Gidley M, Roessner U, Martin C, Smith AM (1999) A combined reduction inactivity of starch synthases II and III of potato has novel effects of the starch of tubers. Plant J., 17, 251-261
Veramendi J, Rössner U, Renz A, Willmitzer L, Trethewey RN (1999) Antisense repression of hexokinase 1 leads to an accumulation of starch in leaves of transgenic potato plants but not to significant changes in tuber carbohydrate metabolism. Plant Physiol., 121, 123-133
Zhu Z-P, Hylton CM, Rössner U, Smith AM (1998) Characterisation of starch-debranching enzymes in pea embryos. Plant Physiol., 118, 581-590

Prof Ute Roessner
School of BioSciences
BioSciences Building 1
The University of Melbourne
3010 Victoria
Australia

Email: u.roessner@unimelb.edu.au

Roessner Lab